The Stereospecificity of the Hill Reaction with Triphosphopyridine Nucleotide
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چکیده
منابع مشابه
The Stereospecificity of the Hill Reaction with Triphosphopyridine Nucleotide.
In 1957, San Pietro and Lang (1) observed that isolated spinach chloroplasts cause a light-stimulated exchange of the hydrogen of water with the hydrogen at the p position of the nicotinamide ring of oxidized TPN and DPN. Their results showed that there are enzymes in chloroplasts which cause activation of both steric positions of the reduced pyridine nucleotides. Since these early studies, it ...
متن کاملThe Stereospecificity of the Hill Reaction with Diphosphopyridine Nucleotide.
Spinach grana cause little photoreduction of DPN unless they are supplemented with ferredoxin and TPN reductase. The latter flavoprotein catalyzes a reduction of DPN+ by TPNH, and has been termed a transhydrogenase on the basis of this reaction (2, 3). The results to be presented will show that the flavoprotein transhydrogenase reaction and the Hill reaction both involve hydrogen addition to th...
متن کاملThe Stereospecificity of Thioredoxin Reductase for Triphosphopyridine Nucleotide.
Escherichia coli B contains a cytidine diphosphate reductase system which utilizes reduced triphosphopyridine nucleotide to effect the reduction of cytidine diphosphate to deoxycytidine diphosphate (1, 2). Two components, thioredoxin and thioredoxin reductase, have been identified as oxidation-reduction carriers between TPNH and cytidine diphosphate. Thioredoxin is a low molecular weight protei...
متن کاملThe measurement of triphosphopyridine nucleotide and reduced triphosphopyridine nucleotide and the role of hemoglobin in producing erroneous triphosphopyridine nucleotide values.
A method is described for determining TPN+ and TPNH in a single tissue homogenate made in dilute NaOH at 0’. One portion, for TPN+, is acidified after adding ascorbic acid to prevent TPNH oxidation. A second portion, for TPNH, is heated (still alkaline) after adding cysteine to prevent oxidation. If desired, a third portion can be analyzed directly without heating for total TPN (TPN+ plus TPNH)...
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abstract while task-based instruction is considered as the most effective way to learn a language in the related literature, it is oversimplified on various grounds. different variables may affect how students are engaged with not only the language but also with the task itself. the present study was conducted to investigate language and task related engagement on the basis of the task typolog...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1965
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)97511-3